Journal: PLoS ONE

Article Title: Control of Neural Stem Cell Survival by Electroactive Polymer Substrates

doi: 10.1371/journal.pone.0018624

Figure Lengend Snippet: (A) In the absence of FGF2, fetal NSCs differentiated into astrocytes (GFAP positive); neurons (marked with Tuj1) and oligodendrocytes (labeled for MBP). PPy(DBS) supported NSC differentiation over 7 days in N2 conditions, similar to TCPS. (B) mRNA expression levels analyzed by real-time PCR showed comparable levels of Nestin, GFAP and ß-tubulin relative to TBP for NSCs when cultured on PPy(DBS) and TCPS for 7 days in N2 media supplemented with FGF2, validated by their immunoreactivity for Nestin. (D) Real-time quantitative PCR results showed similar mRNA levels for Nestin, GFAP and ß-tubulin relative to TBP for fetal NSCs grown on TCPS and PPy(DBS). Student's two-tailed t-test was used. The scale bar represents 100 µm.

Article Snippet: The following primary antibodies and dilutions were used: mouse anti-Nestin from BD Biosciences Pharmingen (1∶500), rabbit anti-glial fibrillary acidic protein (GFAP) from Dako (1∶500), mouse anti-neuronal class III ß-tubulin (Tuj1) from Nordic Biosite (1∶500), rat anti-myelin basic protein (MBP) from Chemicon (1∶250), rabbit anti-fibronectin from Sigma-Aldrich (1∶500) and rabbit anti-brain lipid-binding protein (BLBP) from Millipore (1∶100).

Techniques: Labeling, Expressing, Real-time Polymerase Chain Reaction, Cell Culture, Two Tailed Test

Journal: Anatomical record (Hoboken, N.J. : 2007)

Article Title: Comparative morphology and histology of the nasal fossa in four mammals: gray squirrel, bobcat, coyote and white-tailed deer

doi: 10.1002/ar.23352

Figure Lengend Snippet: Epithelial lining of the bobcat nasal fossa. Sagittal view of segmented MRI scans of the bobcat nasal airway showing five coronal sections (b–f) selected for morphological and histological representation (A). The five coronal sections along the rostrocaudal axis illustrate the maxilloturbinal (B–C), nasomaxillary (D) and ethmoturbinal (E–F) regions of the bobcat nasal fossa. Magnified views show the nasoturbinal (G), maxilloturbinal (H, J) and ethmoturbinal (I) covered with nonsensory epithelium and packed with Alcian blue labeled goblet cells at rostral regions of the nasal fossa. Olfactory epithelium covered the nasoturbinal (K), septum (L), and ethmoturbinal (L) in more caudal regions and had the characteristic epithelial thickness and Bowman’s glands in the lamina propria (M) and labeled with β-tubulin III antibody (N–O). nt = nasoturbinal; mt = maxilloturbinal; s = septum; et = ethmoturbinals; ob = olfactory bulb; oe = olfactory epithelium; bg = Bowman’s gland; ne = nonsensory epithelium. Scale bar: B–F = 2 mm; G–L, N = 100 µm; and M, O = 50 µm.

Article Snippet: To further visualize the OE, we conducted immunohistochemistry with a mouse monoclonal anti-neuronal class III β-tubulin antibody (TMJ1, Babco) as has been used to label olfactory neurons in rodents ( Roskams et al., 1998 ), cats (Lishcka et al., 2008) and humans ( Ronnett et al., 2003 ), suggesting reactivity of this antibody in diverse mammalian samples.

Techniques: Labeling

Journal: Anatomical record (Hoboken, N.J. : 2007)

Article Title: Comparative morphology and histology of the nasal fossa in four mammals: gray squirrel, bobcat, coyote and white-tailed deer

doi: 10.1002/ar.23352

Figure Lengend Snippet: Epithelial lining of the coyote nasal fossa. Sagittal view of segmented MRI scans of the coyote nasal airway showing five coronal sections (b–f) selected for morphological and histological representation (A). The five coronal sections along the rostrocaudal axis illustrate the maxilloturbinal (B–C), nasomaxillary (D) and ethmoturbinals (E–F) regions of the coyote nasal fossa. Nonsensory epithelium covered the nasoturbinal (G), maxilloturbinal (H–K), and ethmoturbinal (L) at the rostral regions. Thick olfactory epithelium (L), clearly labeled with β-tubulin antibody (N–O), covered the septum, nasoturbinal, and ethmoturbinal at the caudal regions of the nasal fossa. nt = nasoturbinal; mt = maxilloturbinal; s = septum; et = ethmoturbinal; fs = frontal sinus; ob = olfactory bulb; oe = olfactory epithelium; ne = nonsensory epithelium. Scale bar: B–F = 2 mm; G–L, N = 100 µm; and M, O = 50 µm.

Article Snippet: To further visualize the OE, we conducted immunohistochemistry with a mouse monoclonal anti-neuronal class III β-tubulin antibody (TMJ1, Babco) as has been used to label olfactory neurons in rodents ( Roskams et al., 1998 ), cats (Lishcka et al., 2008) and humans ( Ronnett et al., 2003 ), suggesting reactivity of this antibody in diverse mammalian samples.

Techniques: Labeling

Journal: Neurobiology of disease

Article Title: Evaluation of the effects of the T-type calcium channel enhancer SAK3 in a rat model of TAF1 deficiency

doi: 10.1016/j.nbd.2020.105224

Figure Lengend Snippet: List of primary and secondary antibodies used in the study.

Article Snippet: Class III β-Tubulin , βIII-Tubulin , Mouse , Promega , G7121 , WB , 1:1000.

Techniques: